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Use of chimeric influenza viruses as a novel internal control for diagnostic rRT-PCR assays

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Wikipedia:Vital articles/List of all articles - Wikipedia

Real-time quantitative reverse transcriptase polymerase chain reaction rRT-PCR is now widely used to detect viral pathogens in various human specimens. The application of internal controls to validate the entire process of these assays is necessary to prevent false-negative results caused by unexpected inhibition or inefficient extraction. In the present study, we describe a strategy to produce a stable internal control for rRT-PCR by packaging foreign RNA into influenza virions using plasmid-based reverse genetics technology. The envelope structure of influenza virus can effectively protect RNA segments from RNase digestion, which provides an advantage for its routine use as an internal control. Moreover, the chimeric influenza virus particles could be easily produced in embryonated eggs and were noninfectious after inactivation treatment. Additionally, this strategy could also be adapted for real-time clinical applications of other RNA targets, providing a universal approach with broad clinical applications in rRT-PCR assays.
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Movies on TV this week: Sunday, Nov. 22, 2020

Movies on TV the week of Nov. Driving Miss Daisy Encore Tues. Encore Wed. Raiders of the Lost Ark Paramount Thur. Paramount Thur.
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Elisabeth Ehmann , Elisabeth Miller. Andrew Annenberg. Bill Clinton. Self Portrait.
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